Evaluation of Antimicrobial Efficacy of Silver Nanoparticles and Chitosan Nanoparticles against Enterococcus Faecalis and Candida Albicans Biofilm (An in vitro Study)

El Wakad, Ahmed Amr; Moukarab, Dalia Ali Ahmed; Abdelhalim, Enas Fathelbab

doi:10.21608/ueccj.2023.256886.1009

Evaluation of Antimicrobial Efficacy of Silver Nanoparticles and Chitosan Nanoparticles against Enterococcus Faecalis and Candida Albicans Biofilm (An in vitro Study)

Document Type : Original Article

Authors

¹ Doctorate Candidate of Endodontics, Faculty of Dentistry, Minia University. Assistant Lecturer of Endodontics, Faculty of Oral and Dental Medicine, Future University in Egypt

² Associate Professor of Endodontics, Head of Endodontic Department Faculty of Dentistry, Minia University

³ Professor of Fixed Prosthodontics Faculty of Dentistry, Minia University

10.21608/ueccj.2023.256886.1009

Abstract

Aim: antibacterial activities of silver nanoparticles and chitosan nanoparticles against Enterococcus Faecalis and Candida Albicans biofilm in an in- vitro model
Material and methods:
Forty extracted human single-rooted permanent teeth were included in the present study. All samples were prepared up to size Revo S (AS 40/0.06), and autoclaved for 20 minutes at 121°C then divided into 5 equal groups (n=8); negative control (no further treatment was preformed) and positive control ( only microbial inoculation was preformed) , all tested groups were inoculated then further treated with irrigation solutions group I (treated with nanosilver particles) , group II ( treated with chitosan nanoparticles ) and group III (treated NaOCl/ EDTA). For all specimens microbial analysis was performed.
Results: The higher median value of bacterial count in Log CFU ml -1 in Positive control was 6.20 (5.99-6.55), followed by Group I (Nano- silver) was 1.38 (0.51-1.88), then Group II (Nano-chitosan) was 1.38 (1.04-1.55), followed by Group III (Sodium hypochlorite) was 0.73 (0.34-1.37), then Negative control was 0 (0-0). Kruskal Wallis test revealed a statistically significant difference between groups (P=0.00). Mann-Whitney test revealed no significant difference between groups I and II, with p-value (p>0.05).
Conclusion: Nano silver and nano chitosan particles are effective microbial agents against E. faecalis and Candida albicans however; sodium hypochlorite has a significantly superior antimicrobial efficacy.

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